Tobacco smoke exacerbates Filifactor alocis pathogenicity.

ABSTRACT:

Aim

Filifactor alocis has recently emerged as a periodontal pathobiont that appears to thrive in the oral cavity of smokers. We hypothesized that identification of smoke-responsive F. alocis genes would provide insight into adaptive strategies and that cigarette smoke would enhance F. alocis pathogenesis in vivo.

Materials and methods

F. alocis was grown in vitro and cigarette smoke extract-responsive genes determined by RNAseq. Mice were exposed, or not, to mainstream 1R6F research cigarette smoke and infected with F. alocis, or not, in an acute ligature model of periodontitis. Key clinical, infectious, and immune data were collected.

Results

In culture, F. alocis growth was unaffected by smoke conditioning and only a small number of genes were specifically regulated

SUBMITTER: Iskander MMZ 

PROVIDER: S-EPMC9976951 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature