Benchmarking of microbiome detection tools on RNA-seq synthetic databases according to diverse conditions

ABSTRACT: Abstract

Motivation

Here, we performed a benchmarking analysis of five tools for microbe sequence detection using transcriptomics data (Kraken2, MetaPhlAn2, PathSeq, DRAC and Pandora). We built a synthetic database mimicking real-world structure with tuned conditions accounting for microbe species prevalence, base calling quality and sequence length. Sensitivity and positive predictive value (PPV) parameters, as well as computational requirements, were used for tool ranking.

Results

GATK PathSeq showed the highest sensitivity on average and across all scenarios considered. However, the main drawback of this tool was its slowness. Kraken2 was the fastest tool and displayed the second-best sensitivity, though with large variance depending on the species to be classified. Th

SUBMITTER:  

PROVIDER: S-EPMC9976984 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature