Rates and spectra of de novo structural mutations in Chlamydomonas reinhardtii.

ABSTRACT: Genetic variation originates from several types of spontaneous mutation, including single-nucleotide substitutions, short insertions and deletions (indels), and larger structural changes. Structural mutations (SMs) drive genome evolution and are thought to play major roles in evolutionary adaptation, speciation, and genetic disease, including cancers. Sequencing of mutation accumulation (MA) lines has provided estimates of rates and spectra of single-nucleotide and indel mutations in many species, yet the rate of new SMs is largely unknown. Here, we use long-read sequencing to determine the full mutation spectrum in MA lines derived from two strains (CC-1952 and CC-2931) of the green alga Chlamydomonas reinhardtii The SM rate is highly variable between strains and between MA lines,

SUBMITTER: Lopez-Cortegano E 

PROVIDER: S-EPMC9977147 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature