Marker-free characterization of full-length transcriptomes of single live circulating tumor cells.

ABSTRACT: The identification and characterization of circulating tumor cells (CTCs) are important for gaining insights into the biology of metastatic cancers, monitoring disease progression, and medical management of the disease. The limiting factor in the enrichment of purified CTC populations is their sparse availability, heterogeneity, and altered phenotypes relative to the primary tumor. Intensive research both at the technical and molecular fronts led to the development of assays that ease CTC detection and identification from peripheral blood. Most CTC detection methods based on single-cell RNA sequencing (scRNA-seq) use a mix of size selection, marker-based white blood cell (WBC) depletion, and antibodies targeting tumor-associated antigens. However, the majority of these methods either miss

SUBMITTER: Poonia S 

PROVIDER: S-EPMC9977151 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature