Distinct roles for canonical and variant histone H3 lysine-36 in Polycomb silencing.

ABSTRACT: Polycomb complexes regulate cell type-specific gene expression programs through heritable silencing of target genes. Trimethylation of histone H3 lysine 27 (H3K27me3) is essential for this process. Perturbation of H3K36 is thought to interfere with H3K27me3. We show that mutants of Drosophila replication-dependent (H3.2^K36R) or replication-independent (H3.3^K36R) histone H3 genes generally maintain Polycomb silencing and reach later stages of development. In contrast, combined (H3.3^K36RH3.2^K36R) mutants display widespread Hox gene misexpression and fail to develop past the first larval stage. Chromatin profiling revealed that the H3.2^K36R mutation disrupts H3K27me3 levels broadly throughout silenced do

SUBMITTER: Salzler HR 

PROVIDER: S-EPMC9977188 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature