Colonic Epithelial Circadian Disruption Worsens Dextran Sulfate Sodium-Induced Colitis.

ABSTRACT:

Background

Disruption of central circadian rhythms likely mediated by changes in microbiota and a decrease in gut-derived metabolites like short chain fatty acids (SCFAs) negatively impacts colonic barrier homeostasis. We aimed to explore the effects of isolated peripheral colonic circadian disruption on the colonic barrier in a mouse model of colitis and explore the mechanisms, including intestinal microbiota community structure and function.

Methods

Colon epithelial cell circadian rhythms were conditionally genetically disrupted in mice: TS4Cre-BMAL1lox (cBMAL1KO) with TS4Cre as control animals. Colitis was induced through 5 days of 2% dextran sulfate sodium (DSS). Disease activity index and intestinal barrier were assessed, as were fecal microbiota and metabolites.

Res

SUBMITTER: Jochum SB 

PROVIDER: S-EPMC9977234 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature