Chloride ions evoke taste sensations by binding to the extracellular ligand-binding domain of sweet/umami taste receptors.

ABSTRACT: Salt taste sensation is multifaceted: NaCl at low or high concentrations is preferably or aversively perceived through distinct pathways. Cl^- is thought to participate in taste sensation through an unknown mechanism. Here, we describe Cl^- ion binding and the response of taste receptor type 1 (T1r), a receptor family composing sweet/umami receptors. The T1r2a/T1r3 heterodimer from the medaka fish, currently the sole T1r amenable to structural analyses, exhibited a specific Cl^- binding in the vicinity of the amino-acid-binding site in the ligand-binding domain (LBD) of T1r3, which is likely conserved across species, including human T1r3. The Cl^- binding induced a conformational change in T1r2a/T1r3LBD at sub- to low-mM concentrations, similar to ca

SUBMITTER: Atsumi N 

PROVIDER: S-EPMC9977269 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature