Spatial transcriptomic characterization of COVID-19 pneumonitis identifies immune circuits related to tissue injury.

ABSTRACT: Severe lung damage resulting from COVID-19 involves complex interactions between diverse populations of immune and stromal cells. In this study, we used a spatial transcriptomics approach to delineate the cells, pathways, and genes present across the spectrum of histopathological damage in COVID-19-affected lung tissue. We applied correlation network-based approaches to deconvolve gene expression data from 46 areas of interest covering more than 62,000 cells within well-preserved lung samples from 3 patients. Despite substantial interpatient heterogeneity, we discovered evidence for a common immune-cell signaling circuit in areas of severe tissue that involves crosstalk between cytotoxic lymphocytes and pro-inflammatory macrophages. Expression of IFNG by cytotoxic lymphocytes was associate

SUBMITTER: Cross AR 

PROVIDER: S-EPMC9977306 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature