CRISPR techniques and potential for the detection and discrimination of SARS-CoV-2 variants of concern.
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ABSTRACT:
SUBMITTER: Xiao H
PROVIDER: S-EPMC9977466 | biostudies-literature | 2023 Apr
REPOSITORIES: biostudies-literature
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CRISPR techniques and potential for the detection and discrimination of SARS-CoV-2 variants of concern.
Xiao Huyan H Hu Jianyu J Huang Camille C Feng Wei W Liu Yanming Y Kumblathan Teresa T Tao Jeffrey J Xu Jingyang J Le X Chris XC Zhang Hongquan H
Trends in analytical chemistry : TRAC 20230302
The continuing evolution of the SARS-CoV-2 virus has led to the emergence of many variants, including variants of concern (VOCs). CRISPR-Cas systems have been used to develop techniques for the detection of variants. These techniques have focused on the detection of variant-specific mutations in the spike protein gene of SARS-CoV-2. These sequences mostly carry single-nucleotide mutations and are difficult to differentiate using a single CRISPR-based assay. Here we discuss the specificity of the ...[more]
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