Bioinformatics Analysis of the Inflammation-Associated lncRNA-mRNA Coexpression Network in Type 2 Diabetes.
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ABSTRACT: Introduction
Diabetes is a chronic inflammatory state, and a key role of lncRNAs in diabetes complications is a new area of research.Methods
In this study, key lncRNAs related to diabetes inflammation were identified by RNA-chip mining and lncRNA-mRNA coexpression network construction and finally verified by RT-qPCR.Results
We ultimately obtained 12 genes, including A1BG-AS1, AC084125.4, RAMP2-AS1, FTX, DBH-AS1, LOXL1-AS1, LINC00893, LINC00894, PVT1, RUSC1-AS1, HCG25, and ATP1B3-AS1. RT-qPCR assays verified that LOXL1-AS1, A1BG-AS1, FTX, PVT1, and HCG25 were upregulated in the HG+LPS-induced THP-1 cells, and LINC00893, LINC00894, RUSC1-AS1, DBH-AS1, and RAMP2-AS1 were downregulated in the HG+LPS-induced THP-1 cells.Conclusions
lncRNAs and mRNAs are extensively linked and form a coexpression network, and lncRNAs may influence the development of type 2 diabetes by regulating the corresponding mRNAs. The ten key genes obtained may become biomarkers of inflammation in type 2 diabetes in the future.
SUBMITTER: Huang L
PROVIDER: S-EPMC9977555 | biostudies-literature | 2023
REPOSITORIES: biostudies-literature
Publications
Bioinformatics Analysis of the Inflammation-Associated lncRNA-mRNA Coexpression Network in Type 2 Diabetes.
Huang Linjuan L Xiong Shengxi S Liu Hanshuang H Li Min M Zhang Ranran R Liu Yan Y Hu Xiaolei X
Journal of the renin-angiotensin-aldosterone system : JRAAS 20230222
<h4>Introduction</h4>Diabetes is a chronic inflammatory state, and a key role of lncRNAs in diabetes complications is a new area of research.<h4>Methods</h4>In this study, key lncRNAs related to diabetes inflammation were identified by RNA-chip mining and lncRNA-mRNA coexpression network construction and finally verified by RT-qPCR.<h4>Results</h4>We ultimately obtained 12 genes, including A1BG-AS1, AC084125.4, RAMP2-AS1, FTX, DBH-AS1, LOXL1-AS1, LINC00893, LINC00894, PVT1, RUSC1-AS1, HCG25, and ...[more]
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