An immunoinformatics approach to study the epitopes of SARS-CoV-2 helicase, Nsp13.

ABSTRACT:

Introduction and objective

Vaccines are administered worldwide to control on-going coronavirus disease-19 (COVID-19) pandemic caused by SARS-CoV-2. Vaccine efficacy is largely contributed by the epitopes present on the viral proteins and their alteration might help emerging variants to escape host immune surveillance. Therefore, this study was designed to study SARS-CoV-2 Nsp13 protein, its epitopes and evolution.

Methods

Clustal Omega was used to identify mutations in Nsp13 protein. Secondary structure and disorder score was predicted by CFSSP and PONDR-VSL2 webservers. Protein stability was predicted by DynaMut webserver. B cell epitopes were predicted by IEDB DiscoTope 2.0 tools and their 3D structures were represented by discovery studio. Antigenicity and allergenicity o

SUBMITTER: Kumar S 

PROVIDER: S-EPMC9977615 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature