Molecular features and evolutionary trajectory of ASCL1⁺ and NEUROD1⁺ SCLC cells.

ABSTRACT:

Background

Small cell lung cancer (SCLC) is the most aggressive subtype of lung cancer without recognised morphologic or genetic heterogeneity. Based on the expression of four transcription factors, ASCL1, NEUROD1, POU2F3, and YAP1, SCLCs are classified into four subtypes. However, biological functions of these different subtypes are largely uncharacterised.

Methods

We studied intratumoural heterogeneity of resected human primary SCLC tissues using single-cell RNA-Seq. In addition, we undertook a series of in vitro and in vivo functional studies to reveal the distinct features of SCLC subtypes.

Results

We identify the coexistence of ASCL1⁺ and NEUROD1⁺ SCLC cells within the same human primary SCLC tissue. Compared with ASCL1⁺ SCLC cell

SUBMITTER: Zhang X 

PROVIDER: S-EPMC9977910 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature