Novel Biomarkers of Health and Degeneration in Human Intervertebral Discs: In-depth Proteomic Analysis of Collagen Framework of Fetal, Healthy, Scoliotic, Degenerate, and Herniated Discs.

ABSTRACT:

Study design

Profiling proteins expressed in the nucleus pulposus (NP) of intervertebral discs (IVDs) in five different biological states.

Purpose

To evaluate the molecular complexity of the collagen (COL) framework and its role in the health and disease of human IVDs.

Overview of literature

Changes in COL composition have been linked to degenerative disk disease (DDD). Despite the fact that humans have 28 different types of COLs, most of the literature focuses solely on COL-1 and COL-2. This study used high-end proteomic technology to examine the entire COL composition of the human IVD across fetal (developmental-FD), normal (healthy-ND), scoliotic (early degeneration-SD), herniated (degenerate-DH), and degenerated (DD) disk phenotypes.

Methods

Forty NP tissue

SUBMITTER: Rajasekaran S 

PROVIDER: S-EPMC9977988 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature