BRCA1 interactors, RAD50 and BRIP1, as prognostic markers for triple-negative breast cancer severity.

ABSTRACT: Introduction: BRIP1 (BRCA1-interacting protein 1) is one of the major interacting partners of BRCA1, which plays an important role in repair by homologous recombination (HR). This gene is mutated in around 4% of cases of breast cancer; however, its mechanism of action is unclear. In this study, we presented the fundamental role of BRCA1 interactors BRIP1 and RAD50 in the development of differential severity in triple-negative breast cancer (TNBC) among various affected individuals. Methods: We have analyzed the expression of DNA repair-related genes in different BC cells using Real-time PCR and western blotting analysis and assessed changes in stemness property and proliferation through Immunophenotyping. We have performed cell cycle analysis to see the defect in checkpoints

SUBMITTER: Katheeja MN 

PROVIDER: S-EPMC9978165 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature