Evaluation of Sampling and Concentration Methods for Salmonella enterica Serovar Typhi Detection from Wastewater.

ABSTRACT: Salmonella enterica serovar (Salmonella Typhi) is the causative bacterial agent of typhoid fever. Environmental surveillance of wastewater and wastewater-impacted surface waters has proven effective in monitoring various pathogens and has recently been applied to Salmonella Typhi. This study evaluated eight sample collection and concentration methods with 12 variations currently being developed and used for Salmonella Typhi surveillance globally to better understand the performance of each method based on its ability to detect Salmonella Typhi and its feasibility. Salmonella Typhi strains Ty21a and Ty2 were seeded to influent wastewater at known concentrations to evaluate the following methods: grab sampling using electropositive filters, centrifugation, direct enrichment, or membrane filt

SUBMITTER: Zhou N 

PROVIDER: S-EPMC9978546 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature