Quantification of 3,4-Dimethyl-1H-Pyrazole Using Ion-Pair LC-MS/MS on a Reversed-Phase Column.

ABSTRACT:

Background

Few methods exist for the analysis of the soil nitrification inhibitor 3,4-dimethyl-1H-pyrazole (3,4-DMP), which is a pesticide with the ability to reduce the production of nitrogenous greenhouse gases in soils as a result of fertilizer application. Due to its small size and polar nature, 3,4-DMP can be difficult to retain on an LC column, which makes diversion of a co-extracted soil matrix away from the MS/MS impossible.

Objective

The current study aims to better control the retention time (RT) of 3,4-DMP. Additionally, 3,4-DMP-15N2 was synthesized and used as an internal standard for the soil extraction of 3,4-DMP.

Methods

Perfluoroalkanoic acids were used as ion-pair reagents and were compared for their abilities to improve peak shape and RT, to better s

SUBMITTER: Doran GS 

PROVIDER: S-EPMC9978597 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature