A tool for evaluating heterogeneity in avidity of polyclonal antibodies.

ABSTRACT: Diversity in specificity of polyclonal antibody (pAb) responses is extensively investigated in vaccine efficacy or immunological evaluations, but the heterogeneity in antibody avidity is rarely probed as convenient tools are lacking. Here we have developed a polyclonal antibodies avidity resolution tool (PAART) for use with label-free techniques, such as surface plasmon resonance and biolayer interferometry, that can monitor pAb-antigen interactions in real time to measure dissociation rate constant (k_d ) for defining avidity. PAART utilizes a sum of exponentials model to fit the dissociation time-courses of pAb-antigens interactions and resolve multiple k_d contributing to the overall dissociation. Each k_d value of pAb dissociation reso

SUBMITTER: Li K 

PROVIDER: S-EPMC9978818 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature