Heme biosensor-guided in vivo pathway optimization and directed evolution for efficient biosynthesis of heme.

ABSTRACT:

Background

Heme has attracted much attention because of its wide applications in medicine and food. The products of genes hemBCDEFY convert 5-aminolevulinic acid to protoporphyrin IX (PPIX; the immediate precursor of heme); protoporphyrin ferrochelatase (FECH) inserts Fe²⁺ into PPIX to generate heme. Biosynthesis of heme is limited by the need for optimized expression levels of multiple genes, complex regulatory mechanisms, and low enzymatic activity; these problems need to be overcome in metabolic engineering to improve heme synthesis.

Results

We report a heme biosensor-guided screening strategy using the heme-responsive protein HrtR to regulate tcR expression in Escherichia coli, providing a quantifiable link between the intracellular heme concentration and cell

SUBMITTER: Zhang J 

PROVIDER: S-EPMC9979517 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature