Complete Workflow for High Throughput Human Single Skeletal Muscle Fiber Proteomics.

ABSTRACT: Skeletal muscle is a major regulatory tissue of whole-body metabolism and is composed of a diverse mixture of cell (fiber) types. Aging and several diseases differentially affect the various fiber types, and therefore, investigating the changes in the proteome in a fiber-type specific manner is essential. Recent breakthroughs in isolated single muscle fiber proteomics have started to reveal heterogeneity among fibers. However, existing procedures are slow and laborious requiring two hours of mass spectrometry time per single muscle fiber; 50 fibers would take approximately four days to analyze. Thus, to capture the high variability in fibers both within and between individuals requires advancements in high throughput single muscle fiber proteomics. Here we use a single cell proteomics meth

SUBMITTER: Momenzadeh A 

PROVIDER: S-EPMC9980124 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature