Human A2-CAR T cells reject HLA-A2+ human islets transplanted into mice without inducing graft versus host disease.

ABSTRACT:

Background

Type 1 diabetes (T1D) is an autoimmune disease characterised by T cell mediated destruction of pancreatic beta-cells. Islet transplantation is an effective therapy, but its success is limited by islet quality and availability along with the need for immunosuppression. New approaches include use of stem cell-derived insulin-producing cells and immunomodulatory therapies, but a limitation is the paucity of reproducible animal models in which interactions between human immune cells and insulin-producing cells can be studied without the complication of xenogeneic graft- versus -host disease (xGVHD).

Methods

We expressed an HLA-A2-specific chimeric antigen receptor (A2-CAR) in human CD4+ and CD8+ T cells and tested their ability to reject HLA-A2+ islets transpla

SUBMITTER: Ellis CE 

PROVIDER: S-EPMC9980131 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature