Identification of astrocyte regulators by nucleic acid cytometry.

ABSTRACT: Multiple sclerosis is a chronic inflammatory disease of the central nervous system¹. Astrocytes are heterogeneous glial cells that are resident in the central nervous system and participate in the pathogenesis of multiple sclerosis and its model experimental autoimmune encephalomyelitis^2,3. However, few unique surface markers are available for the isolation of astrocyte subsets, preventing their analysis and the identification of candidate therapeutic targets; these limitations are further amplified by the rarity of pathogenic astrocytes. Here, to address these challenges, we developed focused interrogation of cells by nucleic acid detection and sequencing (FIND-seq), a high-throughput microfluidic cytometry method that combines encapsulation of cells in droplets, PCR

SUBMITTER: Clark IC 

PROVIDER: S-EPMC9980163 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature