Cerebellar Granule Cells Develop Non-neuronal 3D Genome Architecture over the Lifespan.

ABSTRACT: The cerebellum contains most of the neurons in the human brain, and exhibits unique modes of development, malformation, and aging. For example, granule cells-the most abundant neuron type-develop unusually late and exhibit unique nuclear morphology. Here, by developing our high-resolution single-cell 3D genome assay Dip-C into population-scale (Pop-C) and virus-enriched (vDip-C) modes, we were able to resolve the first 3D genome structures of single cerebellar cells, create life-spanning 3D genome atlases for both human and mouse, and jointly measure transcriptome and chromatin accessibility during development. We found that while the transcriptome and chromatin accessibility of human granule cells exhibit a characteristic maturation pattern within the first year of postnatal life, 3D geno

SUBMITTER: Tan L 

PROVIDER: S-EPMC9980173 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature