Structural and biochemical insights into Zn²⁺-bound EF-hand proteins, EFhd1 and EFhd2.

ABSTRACT: EF-hand proteins, which contain a Ca²⁺-binding EF-hand motif, are involved in regulating diverse cellular functions. Ca²⁺ binding induces conformational changes that modulate the activities of EF-hand proteins. Moreover, these proteins occasionally modify their activities by coordinating metals other than Ca²⁺, including Mg²⁺, Pb²⁺ and Zn²⁺, within their EF-hands. EFhd1 and EFhd2 are homologous EF-hand proteins with similar structures. Although separately localized within cells, both are actin-binding proteins that modulate F-actin rearrangement through Ca²⁺-independent actin-binding and Ca²⁺-dependent actin-bundling activity. Although Ca²⁺ is known to affect the activities of EFhd1 and EFhd2

SUBMITTER: Mun SA 

PROVIDER: S-EPMC9980392 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature