Multiomic spatial analysis reveals a distinct mucosa-associated virome.

ABSTRACT: The human gut virome has been increasingly explored in recent years. However, nearly all virome-sequencing efforts rely solely on fecal samples and few studies leverage multiomic approaches to investigate phage-host relationships. Here, we combine metagenomics, metaviromics, and metatranscriptomics to study virome-bacteriome interactions at the colonic mucosal-luminal interface in a cohort of three individuals with inflammatory bowel disease; non-IBD controls were not included in this study. We show that the mucosal viral population is distinct from the stool virome and houses abundant crAss-like phages that are undetectable by fecal sampling. Through viral protein prediction and metatranscriptomic analysis, we explore viral gene transcription, prophage activation, and the relationship bet

SUBMITTER: Yan A 

PROVIDER: S-EPMC9980608 | biostudies-literature | 2023 Jan-Dec

REPOSITORIES: biostudies-literature