Metabolic heterogeneity and cross-feeding within isogenic yeast populations captured by DILAC.

ABSTRACT: Genetically identical cells are known to differ in many physiological parameters such as growth rate and drug tolerance. Metabolic specialization is believed to be a cause of such phenotypic heterogeneity, but detection of metabolically divergent subpopulations remains technically challenging. We developed a proteomics-based technology, termed differential isotope labelling by amino acids (DILAC), that can detect producer and consumer subpopulations of a particular amino acid within an isogenic cell population by monitoring peptides with multiple occurrences of the amino acid. We reveal that young, morphologically undifferentiated yeast colonies contain subpopulations of lysine producers and consumers that emerge due to nutrient gradients. Deconvoluting their proteomes using DILAC, we find

SUBMITTER: Kamrad S 

PROVIDER: S-EPMC9981460 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature