A large-scale RNAi screen reveals that mitochondrial function is important for meiotic chromosome organization in oocytes.

ABSTRACT: In prophase of the first meiotic division, chromatin forms a compact spherical cluster called the karyosome within the enlarged oocyte nucleus in Drosophila melanogaster. Similar clustering of chromatin has been widely observed in oocytes in many species including humans. It was previously shown that the proper karyosome formation is required for faithful chromosome segregation, but knowledge about its formation and maintenance is limited. To identify genes involved in karyosome formation, we carried out a large-scale cytological screen using Drosophila melanogaster oocytes. This screen comprised 3916 genes expressed in ovaries, of which 106 genes triggered reproducible karyosome defects upon knockdown. The karyosome defects in 24 out of these 106 genes resulted from activation of the meio

SUBMITTER: Nieken KJ 

PROVIDER: S-EPMC9981535 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature