Low-temperature Mossbauer spectroscopy of organs from ⁵⁷Fe-enriched HFE^(-/-) hemochromatosis mice: an iron-dependent threshold for generating hemosiderin.

ABSTRACT: Hereditary hemochromatosis is an iron-overload disease most often arising from a mutation in the Homeostatic Fe regulator (HFE) gene. HFE organs become overloaded with iron which causes damage. Iron-overload is commonly detected by NMR imaging, but the spectroscopic technique is insensitive to diamagnetic iron. Here, we used Mössbauer spectroscopy to examine the iron content of liver, spleen, kidney, heart, and brain of ⁵⁷Fe-enriched HFE^(-/-) mice of ages 3-52 wk. Overall, the iron contents of all investigated HFE organs were similar to the same healthy organ but from an older mouse. Livers and spleens were majorly overloaded, followed by kidneys. Excess iron was generally present as ferritin. Iron-sulfur clusters and low-spin Fe^II hemes (combined into the

SUBMITTER: Vali SW 

PROVIDER: S-EPMC9981716 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature