LINC00659 cooperated with ALKBH5 to accelerate gastric cancer progression by stabilising JAK1 mRNA in an m⁶ A-YTHDF2-dependent manner.

ABSTRACT:

Background

N6-methyladenosine (m⁶ A) RNA modification is known as a common epigenetic regulation form in eukaryotic cells. Emerging studies show that m⁶ A in noncoding RNAs makes a difference, and the aberrant expression of m⁶ A-associated enzymes may cause diseases. The demethylase alkB homologue 5 (ALKBH5) plays diverse roles in different cancers, but its role during gastric cancer (GC) progression is not well known.

Methods

The quantitative real-time polymerase chain reaction, immunohistochemistry staining and western blotting assays were used to detect ALKBH5 expression in GC tissues and human GC cell lines. The function assays in vitro and xenograft mouse model in vivo were used to investigate the effects of ALKBH5 during GC progression.

SUBMITTER: Fang Y 

PROVIDER: S-EPMC9982078 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature