Short term starvation potentiates the efficacy of chemotherapy in triple negative breast cancer via metabolic reprogramming.

ABSTRACT:

Background

Chemotherapy (CT) is central to the treatment of triple negative breast cancer (TNBC), but drug toxicity and resistance place strong restrictions on treatment regimes. Fasting sensitizes cancer cells to a range of chemotherapeutic agents and also ameliorates CT-associated adverse effects. However, the molecular mechanism(s) by which fasting, or short-term starvation (STS), improves the efficacy of CT is poorly characterized.

Methods

The differential responses of breast cancer or near normal cell lines to combined STS and CT were assessed by cellular viability and integrity assays (Hoechst and PI staining, MTT or H₂DCFDA staining, immunofluorescence), metabolic profiling (Seahorse analysis, metabolomics), gene expression (quantitative real-time PCR) and

SUBMITTER: Pateras IS 

PROVIDER: S-EPMC9983166 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature