Unknown

Dataset Information

0

Integrative analysis of green ash phloem transcripts and proteins during an emerald ash borer infestation.


ABSTRACT:

Background

Emerald ash borer (Agrilus planipennis; EAB) is an Asian insect species that has been invasive to North America for 20 years. During this time, the emerald ash borer has killed tens of millions of American ash (Fraxinus spp) trees. Understanding the inherent defenses of susceptible American ash trees will provide information to breed new resistant varieties of ash trees.

Results

We have performed RNA-seq on naturally infested green ash (F. pennsylvanica) trees at low, medium and high levels of increasing EAB infestation and proteomics on low and high levels of EAB infestation. Most significant transcript changes we detected occurred between the comparison of medium and high levels of EAB infestation, indicating that the tree is not responding to EAB until it is highly infested. Our integrative analysis of the RNA-Seq and proteomics data identified 14 proteins and 4 transcripts that contribute most to the difference between highly infested and low infested trees.

Conclusions

The putative functions of these transcripts and proteins suggests roles of phenylpropanoid biosynthesis and oxidation, chitinase activity, pectinesterase activity, strigolactone signaling, and protein turnover.

SUBMITTER: Chiu CC 

PROVIDER: S-EPMC9983263 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Integrative analysis of green ash phloem transcripts and proteins during an emerald ash borer infestation.

Chiu Christine C CC   Pelletier Gervais G   Stival Sena Juliana J   Roux-Dalvai Florence F   Prunier Julien J   Droit Arnaud A   Séguin Armand A  

BMC plant biology 20230303 1


<h4>Background</h4>Emerald ash borer (Agrilus planipennis; EAB) is an Asian insect species that has been invasive to North America for 20 years. During this time, the emerald ash borer has killed tens of millions of American ash (Fraxinus spp) trees. Understanding the inherent defenses of susceptible American ash trees will provide information to breed new resistant varieties of ash trees.<h4>Results</h4>We have performed RNA-seq on naturally infested green ash (F. pennsylvanica) trees at low, m  ...[more]

Similar Datasets

2023-03-08 | GSE212332 | GEO
| MSV000090439 | MassIVE
| PRJNA875060 | ENA
| S-EPMC3174216 | biostudies-literature
| S-EPMC10926479 | biostudies-literature
| S-EPMC6956047 | biostudies-literature
| S-EPMC7610378 | biostudies-literature
| S-EPMC10556020 | biostudies-literature
| S-EPMC3570424 | biostudies-literature