Kif5a Regulates Mitochondrial Transport in Developing Retinal Ganglion Cells In Vitro.

ABSTRACT:

Purpose

Axon transport of organelles and neurotrophic factors is necessary for maintaining cellular function and survival of retinal ganglion cells (RGCs). However, it is not clear how trafficking of mitochondria, essential for RGC growth and maturation, changes during RGC development. The purpose of this study was to understand the dynamics and regulation of mitochondrial transport during RGC maturation using acutely purified RGCs as a model system.

Methods

Primary RGCs were immunopanned from rats of either sex during three stages of development. MitoTracker dye and live-cell imaging were used to quantify mitochondrial motility. Analysis of single-cell RNA sequencing was used to identify Kinesin family member 5A (Kif5a) as a relevant motor candidate for mitochondrial transp

SUBMITTER: Yokota S 

PROVIDER: S-EPMC9983700 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature