Effect of femtosecond laser interaction with human fibroblasts: a preliminary study.

ABSTRACT: In in vitro methods and cell culture models, femtosecond (fs) laser interaction has been employed to assess its effect on the proliferation and morphology of human skin fibroblasts. We cultured a primary human skin fibroblast cell line on a glass plate, passages 17-23. The cells were irradiated with a 90-fs laser at a wavelength of 800 nm and a repetition rate of 82 MHz. The target received an average power of 320 mW for 5, 20, and 100 s, corresponding to the radiation exposures of 22.6, 90.6, and 452.9 J/cm², respectively. Using a laser scanning microscopy technique, the photon densities were measured to be 6.4 × 10¹⁸, 2.6 × 10¹⁹, and 1.3 × 10²⁰ photons/cm² in a spot area of 0.07 cm²; the recorded spectra were obtained from

SUBMITTER: Zaki Ewiss MA 

PROVIDER: S-EPMC9984333 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature