Technical assessment of different extraction methods and transcriptome profiling of RNA isolated from small volumes of blood.

ABSTRACT: Transcriptome profiling of human whole blood is used to discover biomarkers of diseases and to assess phenotypic traits. Recently, finger-stick blood collection systems have allowed a less invasive and quicker collection of peripheral blood. Such non-invasive sampling of small volumes of blood offers practical advantages. The quality of gene expression data is strictly dependent on the steps used for the sample collection, extraction, preparation and sequencing. Here we have: (i) compared the manual and automated RNA extraction of small volumes of blood using the Tempus Spin RNA isolation kit and the MagMAX for Stabilized Blood RNA Isolation kit , respectively; and (ii) assessed the effect of TURBO DNA Free treatment on the transcriptomic data of RNA isolated from small volumes of blood. W

SUBMITTER: Kalikiri MKR 

PROVIDER: S-EPMC9984369 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature