Optical modulation of excitation-contraction coupling in human-induced pluripotent stem cell-derived cardiomyocytes.

ABSTRACT: Non-genetic photostimulation is a novel and rapidly growing multidisciplinary field that aims to induce light-sensitivity in living systems by exploiting exogeneous phototransducers. Here, we propose an intramembrane photoswitch, based on an azobenzene derivative (Ziapin2), for optical pacing of human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). The light-mediated stimulation process has been studied by applying several techniques to detect the effect on the cell properties. In particular, we recorded changes in membrane capacitance, in membrane potential (V_m), and modulation of intracellular Ca²⁺ dynamics. Finally, cell contractility was analyzed using a custom MATLAB algorithm. Photostimulation of intramembrane Ziapin2 causes a transient V_m

SUBMITTER: Vurro V 

PROVIDER: S-EPMC9984557 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature