Application of next-generation metagenomic sequencing in the diagnosis and treatment of acute spinal infections

ABSTRACT:

Objectives

The purpose of this study was to verify the value of metagenomic next-generation sequencing (mNGS) in detecting the pathogens causing acute spinal infection by reviewing the results of mNGS in 114 patients.

Methods

A total of 114 patients were included from our hospital. Samples (tissue/blood) were sent for mNGS detection, and the remaining samples were sent to the microbiology laboratory for pathogen culture, smear, histopathological analysis, and other tests. Patients' medical records were reviewed to determine their rates of detection, time needed, guidance for antibiotic treatment and clinical outcomes.

Results

mNGS showed a satisfying diagnostic positive percent agreement of 84.91% (95% confidence interval (CI): 6.34%–96.7%), compared to 30.19% (9

SUBMITTER:  

PROVIDER: S-EPMC9984843 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature