Linking genotypic and phenotypic changes in the E. coli Long-Term Evolution Experiment using metabolomics.

ABSTRACT: Changes in an organism's environment, genome, or gene expression patterns can lead to changes in its metabolism. The metabolic phenotype can be under selection and contributes to adaptation. However, the networked and convoluted nature of an organism's metabolism makes relating mutations, metabolic changes, and effects on fitness challenging. To overcome this challenge, we use the Long-Term Evolution Experiment (LTEE) with E. coli as a model to understand how mutations can eventually affect metabolism and perhaps fitness. We used mass-spectrometry to broadly survey the metabolomes of the ancestral strains and all 12 evolved lines. We combined this metabolic data with mutation and expression data to suggest how mutations that alter specific reaction pathways, such as the biosynthesis

SUBMITTER: Favate JS 

PROVIDER: S-EPMC9985142 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature