O₂-sensitive microcavity arrays: A new platform for oxygen measurements in 3D cell cultures.

ABSTRACT: Oxygen concentration plays a crucial role in (3D) cell culture. However, the oxygen content in vitro is usually not comparable to the in vivo situation, which is partly due to the fact that most experiments are performed under ambient atmosphere supplemented with 5% CO₂, which can lead to hyperoxia. Cultivation under physiological conditions is necessary, but also fails to have suitable measurement methods, especially in 3D cell culture. Current oxygen measurement methods rely on global oxygen measurements (dish or well) and can only be performed in 2D cultures. In this paper, we describe a system that allows the determination of oxygen in 3D cell culture, especially in the microenvironment of single spheroids/organoids. For this purpose, microthermoforming was use

SUBMITTER: Grun C 

PROVIDER: S-EPMC9986295 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature