Rubredoxin 1 promotes the proper folding of D1 and is not required for heme b₅₅₉ assembly in Chlamydomonas photosystem II.

ABSTRACT: Photosystem II (PSII), the water:plastoquinone oxidoreductase of oxygenic photosynthesis, contains a heme b₅₅₉ iron whose axial ligands are provided by histidine residues from the α (PsbE) and β (PsbF) subunits. PSII assembly depends on accessory proteins that facilitate the step-wise association of its protein and pigment components into a functional complex, a process that is challenging to study due to the low accumulation of assembly intermediates. Here, we examined the putative role of the iron[1Fe-0S]-containing protein rubredoxin 1 (RBD1) as an assembly factor for cytochrome b₅₅₉, using the RBD1-lacking 2pac mutant from Chlamydomonas reinhardtii, in which the accumulation of PSII was rescued by the inactivation of the thylakoid membrane FtsH protease. To this e

SUBMITTER: Calderon RH 

PROVIDER: S-EPMC9986647 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature