Multicolor flow cytometric assessment of Ki67 expression and its diagnostic value in mature B-cell neoplasms.

ABSTRACT:

Background

There is no unified standard data about the sensitivity and specificity regarding flow cytometry analysis of Ki67 expression during lymphoma diagnoses.

Objective

This evaluated the efficacy of multicolor flow cytometry (MFC) in an estimate of the proliferative activity of B-cell non-Hodgkin lymphoma by comparing the expression of Ki67 using MFC and immunohistochemicals (IHC).

Method

A total of 559 patients with non-Hodgkin B-cell lymphoma were immunophenotyped using sensitive MFC, of which 517 were newly diagnosed and 42 were transformed lymphomas. Test samples include peripheral blood, bone marrow, various body fluids, and tissues. Through MFC multi-marker accurate gating, abnormal mature B lymphocytes with restricted expression of the light chain were scr

SUBMITTER: Mao X 

PROVIDER: S-EPMC9986934 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature