PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression.

ABSTRACT:

Background

About 10-20% of patients with bladder cancer (BC) progress to muscle-invasive diseases, of which the underlying key molecular events have yet to be addressed.

Results

Here, we identified poly(A) binding protein nuclear 1 (PABPN1), a general factor of alternative polyadenylation (APA), was downregulated in BC. Overexpression and knockdown of PABPN1 significantly decreased and increased BC aggressiveness, respectively. Mechanistically, we provide evidence that the preference of PABPN1-bound polyadenylation signals (PASs) depends on the relative location between canonical and non-canonical PASs. PABPN1 shapes inputs converging on Wnt signaling, cell cycle, and lipid biosynthesis.

Conclusions

Together, these findings provide insights into how PABPN1-mediated AP

SUBMITTER: Chen L 

PROVIDER: S-EPMC9987104 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature