A transcriptome atlas of leg muscles from healthy human volunteers reveals molecular and cellular signatures associated with muscle location.

ABSTRACT: Skeletal muscles support the stability and mobility of the skeleton but differ in biomechanical properties and physiological functions. The intrinsic factors that regulate muscle-specific characteristics are poorly understood. To study these, we constructed a large atlas of RNA-seq profiles from six leg muscles and two locations from one muscle, using biopsies from 20 healthy young males. We identified differential expression patterns and cellular composition across the seven tissues using three bioinformatics approaches confirmed by large-scale newly developed quantitative immune-histology procedures. With all three procedures, the muscle samples clustered into three groups congruent with their anatomical location. Concomitant with genes marking oxidative metabolism, genes marking fast- o

SUBMITTER: Abbassi-Daloii T 

PROVIDER: S-EPMC9988256 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature