In situ enzymatic generation of Au/Pt nanoparticles as an analytical photometric system: proof of concept determination of tyramine.

ABSTRACT: In situ enzymatic generation of bimetallic nanoparticles, mainly Au/Pt, overcomes the drawbacks (continuous absorbance drift, modest LOQ, and long-time reaction) observed when AuNP alone are produced. In this study, Au/Pt nanoparticles have been characterized by EDS, XPS, and HRTEM images using the enzymatic determination of tyramine with tyramine oxidase (TAO) as a model. Under experimental conditions, the Au/Pt NPs show an absorption maximum at 580 nm which can be related to the concentration of tyramine in the range 1.0 × 10^-6M to 2.5 × 10^-4M with a RSD of 3.4% (n = 5, using 5 × 10^-6M tyramine). The Au/Pt system enables low LOQ (1.0 × 10^-6 M), high reduction of the absorbance drift, and a significant shortening of the reaction time (i.e., from

SUBMITTER: Camacho-Aguayo J 

PROVIDER: S-EPMC9988730 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature