Mouse embryonic stem cell-derived motor neurons are susceptible to ferroptosis.

ABSTRACT: Ferroptosis is a regulated form of cell death driven by the lethal accumulation of lipid peroxides in cell membranes. Several regulators of ferroptosis have been identified using cancer cell lines. However, the cellular pathways of ferroptosis in neurons remain poorly characterized. In this study, we used a mouse embryonic stem cell-derived motor neuron model to investigate how motor neurons respond to ferroptosis inducers. Pharmacological and genetic inhibition of glutathione peroxidase 4 (GPx4) induced ferroptosis in motor neurons, while system x_c ^- inhibition by erastin had no effect. RNA-seq analysis showed that the expression levels of several genes were altered during RSL3-induced ferroptosis. Subsequent bioinformatic analysis revealed alterations in several bio

SUBMITTER: Martinez AM 

PROVIDER: S-EPMC9989922 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature