Enhancement of polyethylene glycol-cell fusion efficiency by novel application of transient pressure using a jet injector.

ABSTRACT: Cell-cell fusion involves the fusion of somatic cells into a single hybrid cell. It is not only a physiological process but also an important cell engineering technology which can be applied to various fields, such as regenerative medicine, antibody engineering, genetic engineering, and cancer therapy. There are three major methods of cell fusion: electrical cell fusion, polyethylene glycol (PEG) cell fusion, and virus-mediated cell fusion. Although PEG cell fusion is the most economical approach and does not require expensive instrumentation, it has a poor fusion rate and induces a high rate of cell cytotoxicity. To improve the fusion rate of the PEG method, we combined it with the pyro-drive jet injector (PJI). PJI provides instant pressure instead of cell agitation to increase the proba

SUBMITTER: Chang CY 

PROVIDER: S-EPMC9989930 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature