Mobile Translation Systems Generate Genomically Engineered Escherichia coli Cells with Improved Growth Phenotypes.

ABSTRACT: Cellular translation is responsible for the synthesis of proteins, a highly diverse class of macromolecules that form the basis of biological function. In Escherichia coli, harnessing and engineering of the biomolecular components of translation, such as ribosomes, transfer RNAs (tRNAs), and aminoacyl-tRNA synthetases, has led to both biotechnology products and an expanded genetic code. However, the engineering potential of molecular translation is hampered by the limited capabilities for rapidly sampling the large genomic space necessary to evolve well-coordinated synthetic translation networks inside cells. To address this limitation, we developed a genome engineering method inspired by the action of mobile genetic elements termed mobilization. Mobilization utilizes the stochastic

SUBMITTER: Gowland S 

PROVIDER: S-EPMC9990117 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature