Extending the Range of SLIM-Labeling Applications: From Human Cell Lines in Culture to Caenorhabditis elegans Whole-Organism Labeling.

ABSTRACT: The simple light isotope metabolic-labeling technique relies on the in vivo biosynthesis of amino acids from U-[¹²C]-labeled molecules provided as the sole carbon source. The incorporation of the resulting U-[¹²C]-amino acids into proteins presents several key advantages for mass-spectrometry-based proteomics analysis, as it results in more intense monoisotopic ions, with a better signal-to-noise ratio in bottom-up analysis. In our initial studies, we developed the simple light isotope metabolic (SLIM)-labeling strategy using prototrophic eukaryotic microorganisms, the yeasts Candida albicans and Saccharomyces cerevisiae, as well as strains with genetic markers that lead to amino-acid auxotrophy. To extend the range of SLIM-labeling applications,

SUBMITTER: Lignieres L 

PROVIDER: S-EPMC9990122 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature