Unique microRNA expression profiles in plasmic exosomes from intrahepatic cholestasis of pregnancy.

ABSTRACT:

Background

Intrahepatic cholestasis of pregnancy (ICP) is strongly associated with an increased risk of adverse perinatal outcomes. Total bile acid (TBA) levels in the late second or third trimester are a major factor in the diagnosis. Here, we sought to establish the miRNA expression profile of plasm exosomes of ICP and identify possible biomarkers for the diagnosis of ICP.

Methods

This case-control study involved 14 ICP patients as the experimental group and 14 healthy pregnant women as the control group. Electron microscopy was used to observe the presence of exosomes in plasma. Nanosight and Western blotting of CD63 was used to assess exosome quality. Among them, three ICP patients and three controls were used for isolation plasmic exosome and preliminary miRNA array ana

SUBMITTER: Kong Y 

PROVIDER: S-EPMC9990296 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature