Integrated analysis of the microbiota-gut-brain axis in response to sleep deprivation and diet-induced obesity.

ABSTRACT:

Introduction

Sleep deprivation (SD) and obesity are common in modern societies. SD and obesity frequently coexist, but research on the combined consequences of SD and obesity has been limited. In this study, we investigated the gut microbiota and host responses to SD and high-fat diet (HFD)-induced obesity. In addition, we attempted to identify key mediators of the microbiota-gut-brain axis.

Methods

C57BL/6J mice were divided into four groups based on whether they were sleep deprived and whether they were fed a standard chow diet (SCD) or HFD. We then performed fecal microbiome shotgun sequencing, gut transcriptome analysis using RNA sequencing, and brain mRNA expression analysis using the nanoString nCounter Mouse Neuroinflammation Panel.

Results

The gut microbiota w

SUBMITTER: Lee J 

PROVIDER: S-EPMC9990496 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature