A MALDI-TOF MS library for rapid identification of human commensal gut bacteria from the class Clostridia.

ABSTRACT:

Introduction

Microbial isolates from culture can be identified using 16S or whole-genome sequencing which generates substantial costs and requires time and expertise. Protein fingerprinting via Matrix-assisted Laser Desorption Ionization-time of flight mass spectrometry (MALDI-TOF MS) is widely used for rapid bacterial identification in routine diagnostics but shows a poor performance and resolution on commensal bacteria due to currently limited database entries. The aim of this study was to develop a MALDI-TOF MS plugin database (CLOSTRI-TOF) allowing for rapid identification of non-pathogenic human commensal gastrointestinal bacteria.

Methods

We constructed a database containing mass spectral profiles (MSP) from 142 bacterial strains representing 47 species and 21 g

SUBMITTER: Asare PT 

PROVIDER: S-EPMC9990839 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature