P16^INK4A flow cytometry of exfoliated cervical cells: Its role in quantitative pathology and clinical diagnosis of squamous intraepithelial lesions.

ABSTRACT:

Background

P16^INK4A is a surrogate signature compensating for the specificity and/or sensitivity deficiencies of the human papillomavirus (HPV) DNA and Papanicolaou smear (Pap) co-test for detecting high-grade cervical squamous intraepithelial lesions or worse (HSIL+). However, traditional p16INK4A immunostaining is labour intensive and skill demanding, and subjective biases cannot be avoided. Herein, we created a high-throughput, quantitative diagnostic device, p16INK4A flow cytometry (FCM) and assessed its performances in cervical cancer screening and prevention.

Methods

P16^INK4A FCM was built upon a novel antibody clone and a series of positive and negative (p16^INK4A -knockout) standards. Since 2018, 24 100-women (HPV-positive/-negative, P

SUBMITTER: He Y 

PROVIDER: S-EPMC9991008 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature